Figure 4
From: Direct PCR with the CDC 2019 SARS-CoV-2 assay: optimization for limited-resource settings
Effect of VTM volume added to an qRT-PCR reaction on performance of primer sets N1 (A) and N2 (B). The addition of 25% VTM was selected as a reasonable balance between the RNA copies introduced to the reaction and the inhibition from VTM for the two primer sets. Each curve is the average of four experiments (N = 4).
