Table 1 Comparison of SARS-CoV-2 detection from unextracted clinical nasopharyngeal specimens using our multiplexed assay optimized for direct qRT-PCR of unextracted specimens compared to the original clinical diagnostic test results using the FDA emergency-use authorized CDC singleplex assay.
From: Direct PCR with the CDC 2019 SARS-CoV-2 assay: optimization for limited-resource settings
Primer/Probe | Positive percent agreement | ΔCt | Negative percent agreement |
|---|---|---|---|
N1 | 92.7% (38/41) | 4.4 ± 0.5 | 100% (43/43) |
N2 | 92.7% (38/41) | 5.8 ± 0.4 | 100% (43/43) |
RP | 86.9% (73/84) | 6.1 ± 0.9 | NA |
