Table 1 Comparison between sequencing and RT-LAMP results for field samples.

From: Detecting lineage-defining mutations in SARS-CoV-2 using colorimetric RT-LAMP without probes or additional primers

Sample1

Ct2

Sequencing Results3

RT-LAMP Results4

Variant

Replicate 1

Replicate 2

Replicate 3

B25

18.17

Alpha

+

B29

16.43

Alpha

B48

20.55

Alpha

B186

20.47

Zeta

B195

19.19

B.1.1

B211

19.58

Zeta

B212

16.32

Gamma

+

+

+

B213

15.07

Gamma

+

+

+

B214

14.46

P.1.12

+

+

+

B215

14.91

Gamma

+

+

+

B216

16.38

P.1.7

+

+

+

B217

14.62

Gamma

+

+

+

B218

12.03

Gamma

+

+

+

B219

22.30

Gamma

+

+

+

B220

14.22

Gamma

+

+

+

B221

14.46

Gamma

+

+

+

B222

21.53

Gamma

+

B223

18.61

Gamma

+

+

+

B224

18.63

Gamma

+

+

+

B225

20.94

Gamma

+

B227

15.92

Gamma

+

+

+

B228

22.38

Gamma

+

B229

19.36

Gamma

+

+

+

B230

18.82

Gamma

+

+

+

B231

18.49

Gamma

+

+

+

B236

21.89

Zeta

B240

18.13

Zeta

B244

20.28

P.7

B245

16.80

P.7

B247

17.17

Gamma

+

+

+

F1

12.89

Gamma

+

+

+

F2

17.51

P.1.7

+

+

+

F3

13.89

Gamma

+

+

+

F4

19.44

P.1.7

+

+

+

F5

17.20

P.1.7

+

+

+

F6

16.30

Gamma

+

+

+

F7

21.98

P.1.7

+

+

+

F8

20.48

P.1.7

+

+

+

F9

21.26

P.1.7

+

+

+

F10

15.92

Gamma

+

+

+

F11

21.97

Delta

F12

21.07

Delta

F13

20.61

Delta

F14

19.29

Delta

F15

18.11

Delta

+

F16

17.77

Delta

+

F17

16.01

Delta

+

+

+

F18

15.09

Delta

+

+

+

F19

15.96

Delta

+

F20

21.31

Delta

F21

22.55

Omicron

F22

21.27

Omicron

F23

20.20

Omicron

F24

19.13

Omicron

F25

17.41

Omicron

F26

21.96

Omicron

F27

20.76

Omicron

F28

19.89

Omicron

F29

18.51

Omicron

F30

15.44

Omicron

  1. The cycle threshold of RT-qPCR was used as a sample selection criterion.
  2. Ct Cycle threshold.
  3. (−) indicates a negative result (no color change).
  4. (+) indicates a positive result (color change).
  5. 1Sample identification code based in the list provided by LGBIO laboratory, UFG (B- samples) or FIOCRUZ (F- samples);
  6. 2The Ct column corresponds to the quantification cycle of the sample positive for SARS-CoV-2 using the 2019nCoV primer kit;
  7. 3Results of sequencing obtained in the GISAID EpiCoV platform;
  8. 4Results of RT-LAMP assay in triplicate;