Table 3 The difference in the melting temperature of St14f between free and complex with StFDL1 peptide measured by thermal shift assay.

From: Crystal structure of potato 14-3-3 protein St14f revealed the importance of helix I in StFDL1 recognition

St14f

ΔTm

SD

WT

4.03

0.20

L229A W232A

0.09a

0.38

S214M

3.60

0.16

S214T

4.01

0.39

S214L

4.26

0.47

Y215A

4.42

0.16

D217A

6.67b

0.17

S218M

7.48b

0.16

S218L

7.61b

0.39

S218G

6.37b

0.81

I221A M222A

4.63

0.03

L229A

6.22b

0.21

  1. aNo direct interaction with the StFDL1 peptide.
  2. bStabilized the complex with the StFDL1 peptide by mutation.
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