Figure 12
Histopathological and histomorphometric assessment using H and E for normal mammary fat pad and Ehrlich induced breast solid tumor in untreated mice and different treated groups with cimetidine at a dose of 200 mg/kg/day, vitamin C at a dose of 4 g/kg/day, and their combination for 15 days. Data are presented as mean ± SD.* Significantly different from the normal control group, P < 0.05. @ is significantly different from Ehrlich untreated group, P < 0.05. $ is significantly different from the vitamin C-treated group, P < 0.05. # is significantly different from the cimetidine-treated group, P < 0.05. (A) A normal control mice breast shows normal breast lobules and ducts. (B) Ehrlich tumor invades the entire breast tissue. (C) Untreated Ehrlich tumor shows numerous mitotic figures. (D) Mild reduction in mitotic figures is shown in breast solid tumor after treatment with vitamin C. (E) Moderate reduction in mitotic figures is shown in breast solid tumor after treatment with cimetidine. (F) The most prominent reduction is shown in breast solid tumor after treatment with the combination of cimetidine and vitamin C. (G) Ehrlich tumor is composed of sheets and masses of large polygonal epithelioid cells, with central areas of necrosis. (H) Demonstrates mild elevation in the extent of necrosis within the tumors after vitamin C treatment. (I) Reveals moderate elevation in the extent of necrosis within the tumors after cimetidine treatment. (J) Indicates the most prominent elevation in the extent of necrosis within the tumors after treatment with the combination therapy.
