Figure 2

Expressions of VE-cadherin (a), ZO-1 (b) and claudin-5 (c) after exposure of cells in our different conditions. For treatment with thrombin, cells were exposed to thrombin at 5 or 10 U/mL for 1 h. Then cells were also exposed to 6 h of IH alone or associated with thrombin at 5 or 10 U/mL during the last hour. Immunostaining of ZO-1 after exposure of cells to our different treatments in our model of blood–brain barrier (d) Scale bar = 100 µm. Results are represented as mean value ± SD (n = 9, N = 3), *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 compared to control conditions.