Table 1 Primers and probes used in the assay, with respective sequences, modifications, and final qPCR concentrations.
Primer/probe | Amplicon size | Sequence/label/modifications | Concentration (nM) |
|---|---|---|---|
CDC-nCOV_N2 Forward | 67 bp | 5’-TTACAAACATTGGCCGCAAA-3’ | 250 |
CDC-nCOV_N2 Reverse | 5’-GCGCGACATTCCGAAGAA-3’ | 250 | |
CDC-nCOV_N2 Probe | 5’-FAM-ACAATTTGC/ZEN/CCCCAGCGCTTCAG-IBFQ-3’ | 62.5 | |
Charité/Berlin E_sarbeco Forward | 113 bp | 5’-ACAGGTACGTTAATAGTTAATAGCGT-3’ | 200 |
Charité/Berlin E_sarbeco Reverse | 5’-ATATTGCAGCAGTACGCACACA-3’ | 200 | |
Charité/Berlin E_sarbeco Probe | 5’-HEX-ACACTAGCC/ZEN/ATCCTTACTGCGCTTCG-IBFQ-3’ | 100 | |
CDC-RNase P Forward | 65 bp | 5’-AGATTTGGACCTGCGAGCG-3’ | 62.5 |
CDC-RNase P Reverse | 5’-GAGCGGCTGTCTCCACAAGT-3’ | 62.5 | |
CDC-RNase P Probe | 5’-ATTO647-TTCTGACCT/ZEN/GAAGGCTCTGCGCG-IBFQ-3’ | 62.5 |
