Table 2 RT-qPCR Cycling conditions.

From: Performance evaluation of a non-invasive one-step multiplex RT-qPCR assay for detection of SARS-CoV-2 direct from saliva

Step

Temperature (°C)

Duration (min:sec)

Cycles

Reverse Transcription

50

10:00

x1

RT Inactivation/Initial Denaturation

95

03:00

x1

Denaturation

95

00:03

x45

Annealing

55

00:30

Extension (Acquisition)

72

00:15

  1. Acquisition of FAM: 510–530 nm; HEX: 560–580 nm; ATTO647 (Cy5 channel): 675–690 nm.
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