Table 5 Descriptive statistics showing the effect of controlled variation of volumes of saliva in a 20 µl reaction.
6 μL | 7 μL | 8 μL | 9 μL | 10 μL | |
|---|---|---|---|---|---|
CDC-N2 (FAM) | |||||
Number of replicates | 3 | 3 | 3 | 3 | 3 |
Mean CT | 33.38 | 33.61 | 32.92 | 33.72 | 34.25 |
Std. Deviation | 0.227 | 0.612 | 0.921 | 1.505 | 0.302 |
Coefficient of variation | 0.680% | 1.821% | 2.796% | 4.463% | 0.882% |
Charité-E (HEX) | |||||
Number of replicates | 3 | 3 | 3 | 3 | 3 |
Mean CT | 32.25 | 32.77 | 32.00 | 32.40 | 33.70 |
Std. Deviation | 0.106 | 0.659 | 0.609 | 1.228 | 0.475 |
Coefficient of variation | 0.329% | 2.011% | 1.902% | 3.791% | 1.408% |
