Figure 5 | Scientific Reports

Figure 5

From: Anxiety-like behavior and microglial activation in the amygdala after acute neuroinflammation induced by microbial neuraminidase

Figure 5

mRNA levels of genes related to neuroinflammation 2 and 10 weeks after the ICV administration of neuraminidase. Animals ICV-injected either with saline (Sal) or with neuraminidase (NA) were sacrificed 2 weeks (2w) or 10 weeks (10w) after the ICV. Gene expression was measured by qPCR in hypothalamic tissue samples. The mRNA levels of the inflammatory cytokine TNFα (A), the inflammasome protein NLRP3 (B), the receptor TLR4 (C) and the alarmin HMGB1 (D) were quantified relative to GAPDH expression. Student’s t-test was performed in order to check the influence of treatment (Sal versus NA) on the expression levels of these genes at both time points. The bars in histograms represent the mean value ± SEM of n = 5–6 animals per experimental group. *p < 0.05.

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