Figure 1
From: Characterization of mitochondrial dysfunction due to laser damage by 2-photon FLIM microscopy
Flow Chart—2-step experimental strategy using a B&H FLIM board coupled to a Zeiss 780 LSM confocal, multiplexing multiphoton and confocal imaging. Following FLIM image acquisition (Step 1) of recorded X–Y–Z positions of different specimens with increasing laser average power in separate experiments, the specimens were labeled individually with different probes for mitochondrial viability and apoptosis and reimaged at identical X–Y–Z positions on the LSM Confocal side (Step 2) with probe specific 1-photon excitation under identical imaging settings to assess laser induced changes in mitochondria post FLIM.
