Figure 1 | Scientific Reports

Figure 1

From: Spautin-1 inhibits mitochondrial complex I and leads to suppression of the unfolded protein response and cell survival during glucose starvation

Figure 1

Spautin-1 inhibits the UPR and cell survival in glucose-starved and 2DG-stressed HT1080 cells. (a) HT1080 cells were transfected with the pGRP78pro160-Luc plasmid and then treated with spautin-1 in the presence of ER stressors (2DG: 10 mM, tunicamycin: 10 μg/mL) for 18 h. Luciferase activity was determined by a dual-luciferase reporter assay system. Data are shown as mean ± SD (n = 4). (b,c) HT1080 cells were treated with spautin-1 or buformin under control or GS conditions for 18 h, followed by western blot analysis for the indicated proteins. RPL7 and RPS3 were used as loading controls. The blot membranes were cut prior to hybridization with antibodies, according to Full range rainbow molecular weight markers. Original blots were presented in Supplementary Fig. S5. (d) HT1080 cells were treated with spautin-1 in the presence of ER stressors (2DG: 10 mM, tunicamycin: 10 μg/mL, thapsigargin: 300 nM) for 48 h. Cell viability was determined by the CellTiter-Glo luminescent cell viability assay. Data are shown as mean ± SD (n = 3).

Back to article page