Table 1 Summary of mutations and their theoretical effect on structure.
Target | Protein/variant name | Theoretical structural effect | Sequence conservation | Structure flexibility | Disulfide bond |
|---|---|---|---|---|---|
Whole protein | Ra-LTP | Unfolded structure | – | – | Yes |
Wt-LTP | Effect of heterologous expression as Trx fusion protein | – | – | – | |
S-S bond | C3S/C50S | Partial unfolding | Yes | ||
C13S/C27S | Yes | ||||
C28S/C73S | Yes | ||||
C48S/C87S | Yes | ||||
S40 & S42 | S40G-S42G | Polar → aliphatic area | Yes | Yes | |
R39 | R39A | No H exchange, contact surface reduced | Yes | Yes | |
R39-AAA-S40 | Local surface alteration | – | Yes | – | |
R39A-D43N | H exchange alteration | Yes | Yes | ||
K72 | K72A | No H exchange, contact surface reduced | Yes | – | – |
N76 | N76A | No H exchange, contact surface reduced | – | Yes | – |
N76-AAA-L77 | Local surface alteration | – | Yes | – | |
D43 | D43N | Charge inversion | Yes | – | – |
D43E-R44K | Contact surface slightly increased | Yes | – | – |
