Table 3 Threshold cycle (Ct) values of PCR analyses of C. jejuni (C.j.) in air samples collected at non-infected and C. jejuni-infected areas in each room.
Sampling day | 17 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Room 1 | ||||||||||||||||||||||
Prevalence | 0% | 0% | 16.6% | 33.3% | 50.0% | 66.6% | 83.3% | 100% | ||||||||||||||
Animal number in box halves | ||||||||||||||||||||||
Non-infected | 12 | 12 | 12 | 12 | 10 | 10 | 10 | 8 | 8 | 8 | 6 | 6 | 6 | 4 | 4 | 4 | 2 | 2 | 2 | 0 | 0 | 0 |
C.j.-infected | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 4 | 4 | 4 | 6 | 6 | 6 | 8 | 8 | 8 | 10 | 10 | 10 | 12 | 12 | 12 |
Ct values | ||||||||||||||||||||||
Non-infected | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 35.4 | – | 36.3 | 36.2 | 37.0 | 34.4 | – | 38.0 |
C.j.-infected | – | – | – | – | – | – | – | – | – | – | 33.4 | 30.9 | 32.5 | 33.6 | 31.1 | 33.5 | 32.1 | 32.5 | 32.5 | 29.4 | 32.4 | 31.1 |
Room 2 | ||||||||||||||||||||||
Prevalence | 0% | 100% | 83.3% | 66.6% | 50.0% | 33.3% | 16.6% | 0% | ||||||||||||||
Animal number in box halves | ||||||||||||||||||||||
C.j.-infected | 12a | 12 | 12 | 12 | 10 | 10 | 10 | 8 | 8 | 8 | 6 | 6 | 6 | 4 | 4 | 4 | 2 | 2 | 2 | 0 | 0 | 0 |
Non-infected | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 4 | 4 | 4 | 6 | 6 | 6 | 8 | 8 | 8 | 10 | 10 | 10 | 12 | 12 | 12 |
Ct values | ||||||||||||||||||||||
C.j.-infected | – | 30.4 | 28.7 | 32.1 | 34.7 | 32.3 | 31.3 | 36.1 | 32.7 | 33.5 | 37.1 | 33.5 | 31.6 | – | 30.1 | 31.3 | 31.2 | 31.1 | 37.0 | 34.6 | – | – |
Non-infected | – | – | 34.2 | – | – | – | 37.9 | – | – | – | – | – | – | 37.4 | 36.4 | – | – | – | – | – | – | – |
