Figure 1 | Scientific Reports

Figure 1

From: Different transcriptional responses by the CRISPRa system in distinct types of heterochromatin in Drosophila melanogaster

Figure 1

Transcription activation of the heterochromatic element TAHRE mediated by the dCas9-VPR. (A) Representation of the TAHRE element present in subtelomeric regions and the location of the designed gRNAs. The position of the gRNAs are indicated in Supp. Table 1. (B) Expression of TAHRE in somatic tissue in adult males (carcasses without testis) and in testis evaluated by RT-PCR. Act5C-GAL4;UAS-dCas9-VPR indicates the RT-PCR from total RNA from flies in which the dCas9 is directed to the TAHRE 5´region compared to the Act5C-GAL4  control flies. rp49 mRNA was used as a loading control. (C) Transcript accumulation of TAHRE RNA in adult testis evaluated by RT-qPCR experiments from two biological replicates. Transcript levels of  rp49  were used as a reference. (D) TAHRE sense and antisence transcripts accumulation. Strand specific RT-qPCR was performed using strand specific primers for the cDNA synthesis (Sup. Table 1).

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