Figure 2
Ubx expression mediated by the dCas9-VPR directed to the Ubx promoter generates ectopic variegated expression causing homeotic transformations. (A) Map of the Ubx gene showing its genomic organization and the position of the gRNAs designed upstream of its promoter. The enhancers localized inside the body of the gene were mapped using previously reported ATAC-seq data49. The genome coordinates of the gRNAs positions are indicated. (B) Phenotypes generated by the ectopic expression of Ubx in adult wings and its corresponding imaginal disc comparing with control lacking dCas9-VPR activator. In the zoom of the pouch it is shown the variegated phenotype in Act5C-GAL4;dCas9-VPR disc. (C) Ectopic expression of Ubx in the eye-antenna disc and the transformed phenotypes from antennas to legs in adult organisms. The identification of Ubx was determined by immunostainings and DNA was stained with DAPI, Barr: 100 μm. The coordinates of the wing and the eye-antenna discs as well as the corresponding adult structures are indicated in the diagrams (for details see the text).
