Figure 3 | Scientific Reports

Figure 3

From: Different transcriptional responses by the CRISPRa system in distinct types of heterochromatin in Drosophila melanogaster

Figure 3

dCas9-VPR directed to the Scr promoter cause the transformation of the legs T2 and T3 into leg T1. (A) Map of the Scr gene showing its genomic organization, the position of enhancers that regulate Scr, as well as the position where the gRNAs were sent upstream of the Scr promoters. The genome coordinates of the gRNAs position is indicated. (B) Ectopic expression of Scr by immunostaining using the actin driver in the T2 and T3 legs discs (Barr: 50 μm.), along with the transformed phenotypes generated in adult legs. The red signal corresponds to Scr and the blue signal are the nuclei visualized by Hoechst-DNA staining. (C) Quantification of the expressivity indicated by the number of extra sex combs in the legs two and three (T2, T3) compared with control Act5C-GAL4 flies.

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