Figure 5
The occupancy of dCas9-VPR in the Scr upstream enhancer cause its ectopic expression and the generation of extra sex combs in T2 and T3 legs , as well as in tarsomeres 2 and 3. (A) Map of the Scr gene showing its genomic organization, the position of enhancers that regulate Scr, as well as the position where the gRNAs were directed in the upstream enhancer. The genome coordinates of the sgRNAs positions are indicated. (B) Extra sex combs phenotypes in male adult legs and the ectopic expression of Scr by immunostaining in leg discs. The arrows indicate the presence of extra sex combs in the tarsomeres T 2 and 3 in T1 and T2. Barr: 50 μm. (C) Quantification of the expressivity indicated by the number of extra sex combs in T2 and T3 compared with Act5C-GAL4 control flies. n = the number of legs analyzed. (D) Comparative table between the homeotic transformation phenotype between organisms in which the dCas9-VPR was sent the promoter region versus when it was sent to the upstream enhancer. The penetrance and the expressivity of the phenotypes are indicated.
