Figure 4 | Scientific Reports

Figure 4

From: Immune modulation by molecularly targeted photothermal ablation in a mouse model of advanced hepatocellular carcinoma and cirrhosis

Figure 4The alternative text for this image may have been generated using AI.

Immunohistochemical analysis of orthotopic HCC tumors treated with MTPA. MTPA was performed in an orthotopic mouse model of HCC with underlying cirrhosis. (A) IHC of CD8+ cells demonstrates minimal infiltrating T cells in control tumors but a substantial increase following anti-PD1 therapy combined with MTPA (red bar = 100 mm). (B) Quantitative analysis of IHC data (error bars reflect standard error) reveals a significant increase in intratumoral CD3+ and CD8+ T cells following MTPA alone and MTPA+anti-PD1 therapy, along with a decrease in CD4+FOXP3+ Tregs. Percentages in panel B reflect the proportion of positively stained cells within a region of interest circumscribing the tumor. Box plots reflect mean +/−  standard error. The non-parametric Mann–Whitney U-test was used to evaluate differences in the proportions across treatment groups.

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