Table 1 Antibodies for leukocyte characterization via FACS.
Antibody | Clone | Fluorochrome | Catalog number | Dilution |
|---|---|---|---|---|
Baseline antibodies | ||||
CD45 | 30-F11 | Alexa Fluor 700 | BioLegend 103128 | 1:100 |
CD3 | 145-2C11 | PerCP-Cy5.5 | BioLegend 100328 | 1:12.5 |
Panels 1 through 3 | ||||
CD4 | RM4-4 | FITC | eBioscience/Invitrogen 11-0043-82 | 1:25 |
CD8a | 53-6.7 | APC-Cy7 | BioLegend 100714 | 1:25 |
Panel 1 | ||||
Ki67* | SolA15 | PE-Cy7 | eBioscience/Invitrogen 25-5698-80 | 1:50 |
Panel 2 | ||||
Granzyme B* | GB11 | Alexa Fluor 647 | BD Pharmingen 560212 | 1:50 |
Interferon gamma* | XMG1.2 | PE | BD Pharmingen 554412 | 1:50 |
Panel 3 | ||||
CD25 | PC61.5 | APC | eBioscience/Invitrogen 17-0251-81 | 1:50 |
FoxP3* | FJK-16 s | PE | eBioscience/Invitrogen 12-5773-80 | 1:50 |
