Figure 1
From: Un-biased housekeeping gene panel selection for high-validity gene expression analysis
Experimental validation of eight housekeeping genes in three different brain ischemia models. (A) Rat hippocampal brain slices were stabilised for 45 min, followed by 15 min of oxygen and glucose deprivation (OGD) and 2 h of re-oxygenation (Re-Ox). (B) Gapdh, Hprt and Rpl13 gene expression remains stable while Ywahz, 18S and Sdha are significantly up-or down-regulated after pharmacological intervention. β2-microglobulin and β-actin showed instability in all hypoxic conditions (*p < 0.05, **p < 0.01, ***p < 0.001, n = 5). (C) After 5 days of culture stabilisation, organotypic hippocampal cultures (OHCs) were exposed to 15 min of OGD followed by 24 h of Re-Ox. (D) While β2-microglobulin, β-actin, Sdha, Hprt and Rpl13 expression remains stable under all experimental conditions, Ywhaz, Gapdh and 18S resulted in unsuitable housekeeping genes for this model (*p < 0.05, **p < 0.01, n = 5). (E) Adult WT mice were subjected to a 1 h-transient occlusion of the middle cerebral artery (tMCAO) followed by 23 h of reperfusion. (F) β2-microglobulin, Sdha, Gapdh, Hprt and Rpl13 gene expression remained stable under all interventions while Ywhaz, 18S and β-actin are significantly up-regulated due to treatment (*p < 0.05, **p < 0.01, ***p < 0.001, n = 5).
