Figure 2
From: Un-biased housekeeping gene panel selection for high-validity gene expression analysis
Opposing gene expression results are based on specific housekeeping gene selection. (A) HK gene validation using acute hippocampal brain slices (HBS) identified Yhawz, \(\beta\)-actin, and 18S as non-stable, while Hprt, Gapdh, and Rpl3 resulted in the best candidates for this model. (B) NADPH oxidase 4 (Nox4) gene expression was assessed in HBSs subjected to 3 different experimental conditions, (i) basal, (ii) oxygen and glucose deprivation (OGD), and (iii) OGD under treatment conditions. Nox4 gene expression was normalised against previously detected unstable genes (Yhawz\(, \beta\)-actin, and 18S) and the most stable candidates (Hprt, Gapdh, and Rpl13). Diametrically opposing results were identified depending on the selected HK gene (#p < 0.05, ##p < 0.01 n = 5) later prevented by 2 h of pharmacological treatment (*p < 0.05, n = 5).
