Figure 3
Application of the PMBN electroporation method to improving delivery of large size DNA, mutagenesis rates and TIS library yields in Gram-negative clinical isolates. (a) Number of UPEC CFT073 electrotransformants with large plasmid constructs recovered from cultures prepared without (Nil) or with PMBN (1 μg/ml). (b) Comparison of putative ΔoafA::cat mutant cfu yields following λ-Red mutagenesis of S. Typhimurium SL1344 electrocompetent cells without (Nil) or with 1 μg/ml PMBN. (c) Comparison of putative transposon mutant cfu yields in UPEC UTI89 following standard electroporation without PMBN (Nil) or with PMBN (3 μg/ml) treatment. In all experiments, the starting competent cell density was the same between groups. Data from three (a,b) or two (c) independent experiments are summarised in graphs showing mean ± SEM.
