Figure 2

T2R38 expression on skin-infiltrating lymphocytes in atopic dermatitis (AD). (A) Representative images of immunohistochemical staining for T2R38 in lesional skin tissue from healthy control and AD. Bars = 100 μm. (B) Quantification of T2R38 positive cells/field in skin tissue. In the cell count of positive cells in immunohistochemical staining, stained cells were counted in 10 random grids under high-power fields. Skin tissue samples were obtained from four patients with AD and four healthy individuals. (C) Representative image of fluorescent double staining for CD3 and T2R38 in lesional skin tissue of AD. Bars = 100 μm. Enlarged images from rectangles in each panels are shown on the right. Bars = 20 μm. T2R38 = red, CD3 = green, DAPI = blue. (D) One representative of two independent experiments is shown. Flow cytometric analysis of T2R38 expression levels in skin-infiltrating lymphocytes in lesional skin AD and PBMC from AD patients. ***P < 0.001 by Student’s t-test.