Figure 1 | Scientific Reports

Figure 1

From: Analysis of three-dimensional chromatin packing domains by chromatin scanning transmission electron microscopy (ChromSTEM)

Figure 1The alternative text for this image may have been generated using AI.

ChromSTEM tomography reconstruction of chromatin in an A549 cell. (A, B) The DRAQ5 photo-oxidation process takes 7 min for each region of interest. Scale bar: 10 µm. (C) The DRAQ5-labeled regions were more intensely stained than the nearby regions (red squares; the letter corresponds to the regions in the left panels). Scale bar: 20 µm. (D) STEM image of a 100 nm thick section of an A549 cell in HAADF mode. Scale bar: 2 µm. (E) Schematics for dual-tilt tomography. The sample was tilted from − 60° to 60° with 2° increments on two perpendicular axes. (F) 3D tomography of the A549 chromatin. Scale bar: 120 nm. (G, H) The fine structure of the chromatin fiber: nucleosomes (blue arrows in G), linker DNA (blue arrows in H) Scale bar: 30 nm. (I, J) 3D rendering of the chromatin organization, the pseudo-color was based on the intensity of the tomograms (Mov. S4–S6). (I) A magnified view of the region labeled by a white square in (J). Pink and green regions represent high and low DNA density regions, respectively.

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