Figure 4
From: An interactive time series image analysis software for dendritic spines
Finding spine neck path in 3D. (A) Two-photon microscopy image of a dendrite of a hippocampal CA1 pyramidal neuron in-vitro. The blue arrow indicates the nearby process. The orange arrow indicates the parent dendrite. The red arrow indicates the spine neck. Image contrast is increased to improve the visibility of the spine neck. (B) ROI in yellow square in (A). The path from the parent dendrite to the spine head border is shown in magenta. The path from the spine head border to the spine head center is shown in green. (C) 3D rendered version of B showing the neck path from three different orientations, showing that the spine neck is correctly traced from the spine center to the dendrite but not to a nearby process.
