Figure 3

B-ALL induces cellular changes in the B-ALL microenvironment that can be normalized by IL-12. Parental BCR-ABL1⁺/Arf^−/− leukemia cells were injected into unirradiated WT recipients. On day 3, treatment with either rIL12 or BSA was begun (1 μg/dose, intraperitoneal, on days 3–7, 10–12); n = 6/group from two independent experiments). (A) Total CD11b⁺CD11c⁺ cells and CD11c⁺B220⁺ cells, as well as total MHCII⁺ cells and CD86 and CD80 mean fluorescence intensity (MFI) of each cell population, respectively. (B) The percentage (top) and total number (bottom) of specified T-cell populations in the bone marrow. The ratio of the number of CD8 to CD4 cells is also shown (right). (ANOVA with Tukey multiple comparison test).