Figure 5
Genes associated with T-cell exhaustion are induced in B-ALL-bearing mice. Murine T-cells were isolated from the bone marrow of either untreated or rIL-12 treated WT mice at day 7 and subjected to targeted RNA sequencing using the nCounter Immune Exhaustion panel (Nanostring). (A) Volcano plots of gene expression changes in T-cells from vehicle treated (top) or IL-12 treated (bottom) mice compared to mice without leukemia. (B) Heat map demonstrating relative gene expression of indicated genes. (C,D) Protein–protein interaction networks with functional annotation from differentially expressed genes (FC > = 1.5, P < 0.05).
