Figure 4

Effect of delivery of miRNAs to downstream target genes. (a) Quantitative PCR results showing in vitro delivery of miRNA loaded PLGA-PEG NPs to U87-MG cells. (b) Western blotting assays to show the effects of downstream mechanisms of the selected target genes. (c) Normalized bioluminescence signals from western blotting assays. The error bars represent the standard deviations of the data. Significance levels are shown in the right margins where, *represent p-value < 0.05. Asterisks show significant difference compared to control cells of the corresponding cell line. Cells = control cells without treatment, 9 = cells treated with condition #9, 10 = cell treated with condition #10.