Figure 4

SHIV DNA in allo-HSC transplanted MCMs. We measured copies of vDNA in (a) PBMCs and (b) peripheral lymph nodes before ART initiation, after ART but before allo-HSCTs, and post-transplant. (c) SHIV DNA levels in the tissues of MCMs B, C, and D at necropsy. Total SHIV DNA was measured by digital droplet PCR using SIV gag-specific primers and normalized to 106 cells by measuring macaque RNaseP p30, RPP30, in companion ddPCR assays. Post-transplant peripheral lymph node samples from MCM-B and MCM-D were unavailable for analysis.