Correction to: Scientific Reports https://doi.org/10.1038/s41598-021-03016-1, published online 28 January 2022


The original version of this Article contained errors in the HPRT Reverse Primer and Probe primer sequences, which should read as: Antisense (HPRT-R) GGTCCTTTTCACCAGCAAGCT and Probe (HPRT-P) CTTTCCTTGGTCAGGCAGTATAATC. As a result, in the Materials and methods section, under the subheading ‘Semi-nested RTqPCR for sample enrichment’,


“The qPCRBIO 1-Step Go Lo-Rox Kit reagents (PCR Biosystems) and manufacturer recommended cycling parameters were used with 900 nM TGACACTGGCAAAACAATGCA HPRT Forward Primer, 900 nM AGCTTGCTGGTGAAAAGGACC HPRT Reverse Primer and 250 nM TTTCCTTGGTCAGGCAGTATAATC VIC/TAMRA Probe (ThermoFisher Scientific).”


now reads:


“The qPCRBIO 1-Step Go Lo-Rox Kit reagents (PCR Biosystems) and manufacturer recommended cycling parameters were used with 900 nM TGACACTGGCAAAACAATGCA HPRT Forward Primer, 900 nM GGTCCTTTTCACCAGCAAGCT HPRT Reverse Primer and 250 nM CTTCCTTGGTCAGGCAGTATAATC VIC/TAMRA Probe (ThermoFisher Scientific).”


Additionally, there was an error in 574P and 599R sequences in Table 2, where “I” should read “N”. Thermofisher does not provide the oigonucletides with an Inosine base. They provide equimolar amounts of each possible base in this position.

574P

LTR 574 ← 552

Probe (total RNA or DNA)

5′-FAM/ACAGAYGGGCACACACIACT/MGBNFQ-3′

599R

LTR 599 ← 582

Reverse primer (total RNA or DNA)

5′-AGGGATCTCTAGITACCA-3′


should read:

574P

LTR 574 ← 552

Probe (total RNA or DNA)

5′-FAM/ACAGAYGGGCACACACNACT/MGBNFQ-3′

599R

LTR 599 ← 582

Reverse primer (total RNA or DNA)

5′-AGGGATCTCTAGNTACCA-3′


The original Article has been corrected.