Figure 1

Exploration of potential isomerizable lipids in eyeballs upon sunlight irradiation. Total ion chromatogram (TIC) was obtained from the (A) nonirradiated and (B) irradiated eyeball extract (data of the first paired sample was shown here). Both positive and negative ion mode data were shown in the TIC. (C) Volcano plots of 1001 identified lipid species in the eyeball extracts. The dashed orange line show where p = 0.05. The dashed purple lines at the right and left show where fold change (irradiated/nonirradiated) = 2 and 0.5, respectively. The ions-of-interest that showed both large magnitude fold changes (x-axis) and high statistical significance (y-axis) were annotated. (D) Distributions of fold changes (irradiated/nonirradiated) of ions associated with CoQ10. A single dot in the dot plot indicates the fold change of one paired sample. The average retention time for [M + H]⁺ and [M + NH₄]⁺ of CoQ10 shown here was 45.69 min and 45.70 min, respectively. CoQ10 Coenzyme Q10, CoQ9 Coenzyme Q9, TG(58:4) triglyceride(58:4).