Table 2 Primer sequences for genes used in confirmatory qRT-PCR.
Gene and ID | Forward primer | Reverse primer | Annealing temperature |
|---|---|---|---|
IL6 | 5′-CCC TGA GGC AAA AGG GAA AGA-3′ | 5′-CTC AGG TGC CCC AGC TAC AT-3′ | 54 |
ANG | 5′-AAT GAA GCA ACG AGG CCT GA-3′ | 5′-GGT TGG ACC CTC CCT TAT GC-3′ | 54 |
ANGPT1 | 5′-TCC ACG CTG AAC GGT TAC AC-3′ | 5′-TTT CCC TCT CAA AGA ACG CC-3′ | 53 |
ET-1 | Proprietary | 55 | |
TGFβ3 | Proprietary | 55 | |
