Figure 1 | Scientific Reports

Figure 1

From: HARLEY mitigates user bias and facilitates efficient quantification and co-localization analyses of foci in yeast fluorescence images

Figure 1

SG quantification is highly variable amongst users and on repetition. Seven users (H, A, B, R, L, M and F) classified foci. Users that did not specify foci sizes are excluded in panels (c) and (e). (a) Example of two ambiguous cells in dataset, with choices of the most permissive (magenta) and most conservative user (teal). (b) Ratio of selected foci to available foci in individual cells by user. (c) Approximate radius in nm of foci by user. Since foci are not perfectly round, the radius corresponds to a circle of the same area as selected foci. (d) Autocorrelation (i.e., user reproducibility) measured by Mathews Correlation Coefficient (MCC; see β€œMaterials and methods”) between two quantification runs on the same dataset. (e) Ratio of foci area differences in second run relative to the first.

Back to article page