Figure 3 | Scientific Reports

Figure 3

From: HARLEY mitigates user bias and facilitates efficient quantification and co-localization analyses of foci in yeast fluorescence images

Figure 3

Intensities and curvatures (not to scale) of artificial and real foci with resulting images. (a,b) Foci modelled by different functions in red and their second derivative in blue (not to scale). The top row represents a slice through the intensity along the radius and has the inflection point (magenta) and the curvature maximum (green) marked. These points correspond to outlines of foci depicted as circles of the same color in bottom row. (c) Real life example from our dataset with multiple inflection points. Yellow lines represent inner and outer contours of the foci candidate \({C}_{k}\) and \({C}_{j}\) respectively.

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