Figure 4

cryoDRGN heterogeneous reconstruction of SAGA. (a) CryoDRGN reconstructed density maps of SAGA in “donut”, “curved”, and “arched” conformations. Visualized density maps were generated at the 5th, 50th, and 95th percentile along the principal component (PC) 2 axis of the dataset’s latent embeddings. The view of the SAGA maps is rotated 180° compared to the density maps in Fig. 2a to allow better visualization of the tail region. Note that the prominent DUB module cannot be visualized in this orientation and the TBP density is also slightly obstructed. (b) PCA projection of the latent embeddings of SAGA particle images. Points depict a traversal of latent embeddings along the PC2 axis corresponding to density maps in (a) and Supplementary Video S1. (c) Clustering of the cryoDRGN volume ensemble. The three largest clusters are labeled according to the associated conformation of SAGA. (d) Number of particles in each cluster. All figures were prepared using cryoDRGN²⁶.