Figure 7
Schematic template of a typical MEBPVC to generate variants from REF_SEQ. The MEBPVC contains five major components: (1) N-terminal adjuvant (blue color), (2) predicted MHC I epitopes (green color border, position number 1–5, TLDSKTQSL, GKQGNFKNL, CYGVSPTKL, KIADYNYKL, VVVLSFELL), (3) predicted MHC II epitopes (magenta color border, position number 6–10, IGINITRFQ, YGFQPTNGV, VLSFELLHA, LQIPFAMQM, IAIVMVTIM), (4) linkers AAY (orange color), GPGPG (cyan color), (5) C-terminal HIS-tag (red color). The MEBP variants were generated by shuffling the epitopes at the ten positions.
