Figure 4 | Scientific Reports

Figure 4

From: Development and characterization of a camelid derived antibody targeting a linear epitope in the hinge domain of human PCSK9 protein

Figure 4

The SDS-PAGE gel of multiple VHH-B11 derived antibodies. The SDS–PAGE gel of multiple VHH-B11 derived antibodies. M1/M2/M3: the protein markers (Fermentas, USA). Lane 1: The VHH-B11 expressed by P. pastoris X33 (pPICZα); lane 2: The bivalent VHH-(G4S)3-VHH expressed by Pichia pastoris X33 (pPICZα); lane B: blank control; lane 4: the HEK293F culture medium superposition of the trivalent antibody VHH-(G4S)3-VHH-(G4S)3-VHH; lane 5: the Ni+ affinity column flow-through of the trivalent antibody VHH-(G4S)3-VHH-(G4S)3-VHH; lane 6: the purified trivalent antibody VHH-(G4S)3-VHH-(G4S)3-VHH; lane7: Llama VHH-human IgG4 hinge linker-IgG4 Fc chimeric HcAb (VHH-Fc); lane 8: the tetravalent VHH-llama long linker-VHH-human IgG4 hinge linker-IgG4 Fc (VHH-VHH-Fc); lane 9: the tetravalent VHH-(G4S)3-VHH-human CH1-IgG4 hinge linker- IgG4 Fc chimeric Ab (VHH-VHH-CH1-Fc); lane 10: the tetravalent VHH-human IgG4 hinge linker-IgG4 Fc-llama long linker-VHH chimeric Ab (VHH-Fc-VHH).

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