Figure 3 | Scientific Reports

Figure 3

From: Identification of small molecules targeting homoserine acetyl transferase from Mycobacterium tuberculosis and Staphylococcus aureus

Figure 3

(A,B) Preliminary screening of HSAT activity using small molecules belonging to National Cancer Institute-Developmental Therapeutic Program Library. (A) The entire NCI-DTP library comprising of 2443 compounds belonging to either diversity set (A) and mechanistic set (B) was screened at 100 µM concentration to identify inhibitors for Rv3341. The data shown in this panel is percentage inhibition obtained from a single experiment. (C,D) HSAT enzymatic inhibition by the identified primary hits. The homoserine acetyl transferase activity associated with HSAT homologs from either M. tuberculosis (C) or S. aureus (D) was measured in the presence of identified primary hits at 100 µM concentration. The data shown in these panels is mean ± S.E. of percentage inhibition obtained from three independent experiments performed in duplicates.

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