Figure 3 | Scientific Reports

Figure 3

From: Sirtuin 5 levels are limiting in preserving cardiac function and suppressing fibrosis in response to pressure overload

Figure 3

Transcriptomic analysis of heart tissue four weeks after TAC identifies a dampened transcriptional response to pressure overload in SIRT5OE mice. (A) Principal component analysis (PCA) of RNA-sequencing data of the four groups of mice [WT sham (n = 4), WT TAC (n = 4), SIRT5OE sham (n = 3) and SIRT5OE TAC (n = 4)]. (B) Hierarchical clustering analysis (HCA) of the RNA-seq data based on the top 30 genes, determined by greatest variance from the mean. Genes with higher expression compared to the mean skew red; genes with lower expression compared to the mean skew blue. (C) Postn, Spp1, and Thbs1 mRNA levels measured by RNA-seq. (D) Venn Diagram comparing exclusive and overlapping differentially expressed genes in the WT (sham vs TAC) mice and the SIRT5OE (sham vs TAC) mice. (E–G) Top five positively and negatively enriched Gene Ontology (GO) pathways from the indicated comparisons, determined by gene set enrichment analysis (GSEA). NES: normalized enrichment score. All listed pathways were significantly enriched with a false discovery rate (FDR) < 0.0001.

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