Figure 3 | Scientific Reports

Figure 3

From: A PCR-lateral flow immunochromatographic assay (PCR-LFA) for detecting Aristolochia species, the plants responsible for aristolochic acid nephropathy

Figure 3

The primer design for the detection of Aristolochia spp. by PCR-LFA on a partial rbcL gene alignment. Selected plant species are shown in the alignment. Primers A397F (red) and R502 (green) were used for amplification of the internal control amplicon. Primer C357F (blue and R502 (green) was used for amplification of the Aristolochia-specific PCR amplicon. At the 5'-end of C357F, R502 and A397F primers were labeled with FAM (F), biotin (B) and DIG (D), respectively. The yellow box and asterisk present the nucleotide signature of the Aristolochia plant. Consensus sequences are indicated with dots. The altered bases indicate the sequence differences.

Back to article page