Figure 1
Detection of TRPA1, TRPV1, and TRPV4 in undifferentiated hDPSC-K4DT cells. (A) Relative mRNA expression levels of TRPA1, TRPV1, and TRPV4 in undifferentiated hDPSC-K4DT (white bars) and HCEM cells (gray bars), which were analyzed by RT-qPCR. GAPDH was used as the reference gene. The data are presented as the mean ± SD (n = 3). # indicates a significant difference (P < 0.05) between TRPA1 and TRPV1, or TRPV4 in undifferentiated hDPSC-K4DT cells by using Dunnett's post hoc test following one-way ANOVA. ** indicates a significant difference (P < 0.01) in relative expression levels of TRPA1, TRPV1, and TRPV4 between undifferentiated hDPSC-K4DT and HCEM cells using Student’s t-test. (B) Osteogenic differentiation enhances TRP channel expression in hDPSC-K4DT cells. Relative mRNA expression levels of TRPA1, TRPV1, and TRPV4 in hDPSC-K4DT cultured with the osteoinduction medium for osteogenic differentiation at 0, 8, and 15 days were measured using qRT-PCR. The data are presented as the mean ± SD (n = 3). Differences were assessed by Dunnett's post hoc test following one-way ANOVA. #P < 0.05 compared with 0 day. (C) Detection of osteoblastic markers, COLA1 and ALP. Relative mRNA expression levels of COLA1 and ALP at 0 (white bars) and 15 (black bars) days after osteogenic differentiation of hDPSC-K4DT cells were measured using qRT-PCR. The data are presented as the mean ± SD (n = 3). Differences were assessed by Student’s t-test. **P < 0.01.
