Figure 5
Time- and concentration-dependent effects of HEMA on HO-1 expression levels. (A) Concentration-dependent effect of HEMA on HO-1 expression levels. The hDPSC-K4DT cells at 14 days after osteogenic differentiation were incubated for 3 h with several concentrations (0–30 mM) of HEMA, and subjected to RT–qPCR for determining relative mRNA expression levels of HO-1. (B) RT-qPCR analysis for HEMA-induced HO-1 after administration of free radical scavengers. We assessed the HEMA-induced HO-1 expression levels in osteodifferentiated hDPSC-K4DT cells pretreated for 1 h with 6 mM PBN followed by 3 h co-incubation with HEMA. The data are presented as the mean ± SD (n = 3). #P < 0.05, Student’s t-test. (C) Time-dependent effects of HEMA on HO-1 expression levels. The hDPSC-K4DT cells were culture in normal or osteogenic induction medium for 15 days, and then incubated with HEMA (3 mM) for the indicated periods, and RT–qPCR was used to determine relative mRNA expression levels of HO-1, (D) TRPA1, (E) COLA1, and (F) ALP. GAPDH was used as the reference gene. The data are presented as the mean ± SD (n = 3–6). Differences were assessed by Dunnett's test. *p < 0.05 vs. no differentiation groups.
