Figure 5

Effect of perinatal stress on pancreatic tissue Bip (A), Chop (B), and WFS1 (C) mRNA expression and pancreatic extracted ER levels of Bip (D,E), Chop (D,F) and WFS1 (D,G) proteins in young adult male offspring. Representative figures for the RT-PCR are shown the fold change (3 rats/group, 3 litters/group) and representative bands are shown the respective densitometric values (8 rats/ group, 8 litters/group). Twenty μg proteins were separated on SDS-PAGE, western blotted, probed with anti-Bip antibody, anti-Chop antibody, and anti-WFS1 antibody and reprobed with anti-Calnexin antibody (D) The densities of proteins bands are measured, and the ratio is calculated (E–G). Each column represents mean ± SEM. Data was analyzed using one-way ANOVA followed by Tukey’s post hoc test; ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 versus CTRL group; ⁺P < 0.05, ⁺⁺P < 0.01, ⁺⁺⁺P < 0.001 versus PPPLS group; ^ɛP < 0.05, ^ɛɛP < 0.01 versus PLS group; ^δP < 0.05, ^δδP < 0.01 versus PPLS group. CTRL non stress, PPPLS pre-pregnancy, pregnancy, lactation stress, PPS pre-pregnancy stress, PS pregnancy stress, LS lactation stress, PPPS pre-pregnancy, pregnancy stress, PLS pregnancy, lactation stress, PPLS pre-pregnancy, lactation stress.