Table 2 Primers used for real-time PCR analysis.

From: Maternal stress induced endoplasmic reticulum stress and impaired pancreatic islets’ insulin secretion via glucocorticoid receptor upregulation in adult male rat offspring

Primer name

Gene bank accession no

Primer sequence (5′→3ʹ)

WFS1

NM_ 031,823.1

Forward: GACAAGATTGAACCGCCTCGT

Reverse: CCACATCTGCCTTCATGGGAC

Bip

NM _ 013,083.2

Forward: CAATGACCAAAACCGCCTGA

Reverse: GCTTTCCAATTCATTCCTCGT

Chop

NM_001109986.1

Forward: ATGAACTGTTGGCATCACCT

Reverse: TGCACTGGAGATTACATGCTT

GR

NM_012576.2

Forward: AGGCTTCAGAAACTTACACC

Reverse: CAAATGCCATGAGAAACATCC

ß-Actin

NM_31144.3

Forward: CTCATGAAGATCCTGACCGAG

Reverse: TCTCTTTAATGTCACGCACGA

  1. WFS1 wolframin ER transmembrane Glycoprotein, Bip (Hsp70) heat shock protein family A member 4, Chop (Ddit3) DNA-damage inducible transcript3, GR (Nr3c1) nuclear receptor subfamily 3, group C, member 1.
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