Table 3 Primers used for MSP methylation.

From: Maternal stress induced endoplasmic reticulum stress and impaired pancreatic islets’ insulin secretion via glucocorticoid receptor upregulation in adult male rat offspring

Primer name

Product size (bp)

Primer sequence (5′→3ʹ)

WFS1 methylation (M)

199

Forward: GCGTTTATAGGGAAAGAATGTC

Reverse: ACACAAAACACTAAAAACTTCACGAC

WFS1 unmethylation (U)

200

Forward: GGTGTTTATAGGGAAAGAATGTT

Reverse: ACACAAAACACTAAAAACTTCACAACT

Back to article page