Figure 1
CD47 is highly expressed in renal cell carcinoma (RCC) and is associated with a worse prognosis. (a) A comparison of PD-L1 and CD47 expression levels in patients with RCC (n = 1,017) using TCGA data. The box and whisker plot shows the median, minimum, and maximum values. ****P < 0.0001 (two-tailed t test). (b) Kaplan–Meier overall survival curves of patients with clear cell RCC (n = 526) according to differential expression (median) of PD-L1 or CD47. Analysis was performed with TCGA data. P values were calculated by log-rank test. (c) A comparison of PD-L1 and CD47 mRNA expression levels in human RCC cell lines (n = 31) using CCLE data. The box and whisker plot shows the median, minimum, and maximum values. ****P < 0.0001 (two-tailed t test). (d) A comparison of surface PD-L1 and CD47 expression levels in human renal cell carcinoma cell lines (n = 8) analyzed by flow cytometry. Box and whisker plot (left) shows the mean, minimum, and maximum values in all RCC cell lines. Bar graph (right) represents Δ geometric mean fluorescence intensity (MFI) ± SD of each RCC cell line averaged from three independent experiments. The Δ geometric MFI was calculated by subtracting the MFI of stained cells with isotype control from the MFI of cells stained with the antibody. (e) Representative histogram indicating ‘d’ shows isotype control (gray-filled) and staining (blue-filled) with anti-PD-L1 antibody (left) or anti-CD47 antibody (right).
