Figure 4
VEGFR TKIs affect the susceptibilities of RCC cell lines to macrophages. (a) Graph summarizing the fold change of altered trogocytosis by each VEGFR TKI in all RCC cell lines for each drug (n = 4). All RCC cells were exposed to each VEGFR TKI at a concentration of 10 μM for 24 h before the phagocytosis assay. (b) Trogocytosis (%) analysis of macrophages cocultured with each RCC cell line exposed to each VEGFR TKI for 24 h in the presence of IgG4 isotype control or IMC-002 (n = 3 ~ 5). Phagocytosis assay was analyzed using flow cytometry after coculture of tumor cells and macrophages for 2 h at an E:T ratio of 1:1. Dimethyl sulfoxide solution was used as a control.
