Figure 2 | Scientific Reports

Figure 2

From: Production of kidney organoids arranged around single ureteric bud trees, and containing endogenous blood vessels, solely from embryonic stem cells

Figure 2

Differentiation of mESC to nephron and stromal progenitors (iNP, iSP). (a) Representative images of embryoid bodies before (48 h) and after differentiation (228 h). Scale bar, 200 µm. (b) Immunostaining for endothelial (CD31), ureteric bud (CK8), nephron progenitor (SIX2) and stromal progenitors (MEIS1) in differentiated embryoid bodies. n = 5/5. Scale bar, 100 µm. (c) Representative flow cytometry data of E11.5 kidney and differentiated embryoid bodies (mESC NP/SP Diff) showing the ITGA8high/PDGFRAlow gate for NP/iNP cells and the INTGA8low / PDGFRAhigh gate for SP/iSP) cells. Ureteric bud (EpCAM/RET + ve) and endothelial cells (CD31) were negatively gated before sorting NP/iNP and SP/iSP cells. Abbreviations: EC: endothelial cells, UB: ureteric bud, iELC: induced endothelial like cells, eUBLC: engineered ureteric bud like cells. (d) Immunostaining for MEIS1 and FOXD1 in sorted iSP cells. n = 3/3. Scale bar, 200 µm.

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