Figure 4 | Scientific Reports

Figure 4

From: Production of kidney organoids arranged around single ureteric bud trees, and containing endogenous blood vessels, solely from embryonic stem cells

Figure 4

Ureteric bud tips contribute to ex-fetu organoids. (a) Schematic of the experiment: sorted nephron progenitor (NP) and stromal progenitors (SP) were aggregated with either whole UB (T shape) or UB tip. (b) Schematic summary of the natural expression of markers used in C. (c) (1) Bright field and (2–4) immunofluorescence images showing arborization of UB and formation of nephrons and epithelia, whether the organoids began with an intact UB or a UB tip. Scale bar, 200 µm for bright field images and others 100 µm. CD31: Platelet endothelial cell adhesion molecule, CK8: cytokeratin 8, WTI: Wilms tumor 1, JAG: jagged1, PCK: pan-cytokeratin, POD: podocalyxin, URO: uroplakin. Images are representative of at least three different experiments.

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